Acylgalactosylglycerols as a source of long-chain fatty acids for a naturally occurring rumen auxotroph [proceedings].

detected with sodium dodecyl sulphate/polyacrylamide slab gels. This material, which has an apparent mol.wt. of at least 500000, is stained by the periodic acid/Schiff reagent. Preliminary studies show that galactose is incorporated into this substance most rapidly in the early stages of culture (0-36h) and again after fusion has occurred (6096 h). Other workers have reported that the large, extracellular transformation-sensitive protein is present in myoblasts (Chen, 1977). This material has a mol.wt. of 250000, however, and it is not yet clear whether the macromolecular acceptor for galactose is an aggregate of this membrane glycoprotein, or another type of proteoglycan.